Bradyrhizobium agreste sp. nov., Bradyrhizobium glycinis sp. nov. and Bradyrhizobium diversitatis sp. nov., isolated from a biodiversity hotspot of the genus Glycine in Western Australia.

ABSTRACT: Strains of the genus Bradyrhizobium associated with agronomically important crops such as soybean (Glycine max) are increasingly studied; however, information about symbionts of wild Glycine species is scarce. Australia is a genetic centre of wild Glycine species and we performed a polyphasic analysis of three Bradyrhizobium strains-CNPSo 4010^T, CNPSo 4016^T, and CNPSo 4019^T-trapped from Western Australian soils with Glycine clandestina, Glycine tabacina and Glycine max, respectively. The phylogenetic tree of the 16S rRNA gene clustered all strains into the Bradyrhizobium japonicum superclade; strains CNPSo 4010^T and CNPSo 4016^T had Bradyrhizobium yuanmingense CCBAU 10071^T as the closest species, whereas strain CNPSo 4019^T was closer to Bradyrhizobium liaoningense LMG 18230^T. The multilocus sequence analysis (MLSA) with five housekeeping genes-dnaK, glnII, gyrB, recA and rpoB-confirmed the same clusters as the 16S rRNA phylogeny, but indicated low similarity to described species, with nucleotide identities ranging from 93.6 to 97.6% of similarity. Considering the genomes of the three strains, the average nucleotide identity and digital DNA-DNA hybridization values were lower than 94.97 and 59.80 %, respectively, with the closest species. In the nodC phylogeny, strains CNPSo 4010^T and CNPSo 4019^T grouped with Bradyrhizobium zhanjiangense and Bradyrhizobium ganzhouense, respectively, while strain CNPSo 4016^T was positioned separately from the all symbiotic Bradyrhizobium species. Other genomic (BOX-PCR), phenotypic and symbiotic properties were evaluated and corroborated with the description of three new lineages of Bradyrhizobium. We propose the names of Bradyrhizobium agreste sp. nov. for CNPSo 4010^T (=WSM 4802^T=LMG 31645^T) isolated from Glycine clandestina, Bradyrhizobium glycinis sp. nov. for CNPSo 4016^T (=WSM 4801^T=LMG 31649^T) isolated from Glycine tabacina and Bradyrhizobium diversitatis sp. nov. for CNPSo 4019^T (=WSM 4799^T=LMG 31650^T) isolated from G. max.

SUBMITTER: Klepa MS 

PROVIDER: S-EPMC8375429 | biostudies-literature |

REPOSITORIES: biostudies-literature