Chronic Manganese Exposure and the Enteric Nervous System: An in Vitro and Mouse in Vivo Study.

ABSTRACT:

Background

Chronic environmental exposure to manganese (Mn) can cause debilitating damage to the central nervous system. However, its potential toxic effects on the enteric nervous system (ENS) have yet to be assessed.

Objective

We examined the effect of Mn on the ENS using both cell and animal models.

Method

Rat enteric glial cells (EGCs) and mouse primary enteric cultures were exposed to increasing concentrations of Mn and cell viability and mitochondrial health were assessed using various morphological and functional assays. C57BL/6 mice were exposed daily to a sublethal dose of Mn (15mg/kg/d) for 30 d. Gut peristalsis, enteric inflammation, gut microbiome profile, and fecal metabolite composition were assessed at the end of exposure.

Results

EGC mitochondri

SUBMITTER: Ghaisas S 

PROVIDER: S-EPMC8375672 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature