ZWA: Viral genome assembly and characterization hindrances from virus-host chimeric reads; a refining approach.

ABSTRACT: Viral metagenomics, also known as virome studies, have yielded an unprecedented number of novel sequences, essential in recognizing and characterizing the etiological agent and the origin of emerging infectious diseases. Several tools and pipelines have been developed, to date, for the identification and assembly of viral genomes. Assembly pipelines often result in viral genomes contaminated with host genetic material, some of which are currently deposited into public databases. In the current report, we present a group of deposited sequences that encompass ribosomal RNA (rRNA) contamination. We highlight the detrimental role of chimeric next generation sequencing reads, between host rRNA sequences and viral sequences, in virus genome assembly and we present the hindrances these reads may

SUBMITTER: Dovrolis N 

PROVIDER: S-EPMC8376068 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature