A Cre-Dependent CRISPR/dCas9 System for Gene Expression Regulation in Neurons.

ABSTRACT: Site-specific genetic and epigenetic targeting of distinct cell populations is a central goal in molecular neuroscience and is crucial to understand the gene regulatory mechanisms that underlie complex phenotypes and behaviors. While recent technological advances have enabled unprecedented control over gene expression, many of these approaches are focused on selected model organisms and/or require labor-intensive customization for different applications. The simplicity and modularity of clustered regularly interspaced short palindromic repeats (CRISPR)-based systems have transformed genome editing and expanded the gene regulatory toolbox. However, there are few available tools for cell-selective CRISPR regulation in neurons. We designed, validated, and optimized CRISPR activation (CRISPRa)

SUBMITTER: Carullo NVN 

PROVIDER: S-EPMC8376295 | biostudies-literature | 2021 Jul-Aug

REPOSITORIES: biostudies-literature