The mechanism of action of the SSB interactome reveals it is the first OB-fold family of genome guardians in prokaryotes.

ABSTRACT: The single-stranded DNA binding protein (SSB) is essential to all aspects of DNA metabolism in bacteria. This protein performs two distinct, but closely intertwined and indispensable functions in the cell. SSB binds to single-stranded DNA (ssDNA) and at least 20 partner proteins resulting in their regulation. These partners comprise a family of genome guardians known as the SSB interactome. Essential to interactome regulation is the linker/OB-fold network of interactions. This network of interactions forms when one or more PXXP motifs in the linker of SSB bind to an OB-fold in a partner, with interactome members involved in competitive binding between the linker and ssDNA to their OB-fold. Consequently, when linker-binding occurs to an OB-fold in an interactome partner, proteins are loaded

SUBMITTER: Bianco PR 

PROVIDER: S-EPMC8376408 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature