Biochemistry, structure, and cellular internalization of a four nanobody-bearing Fc dimer.

ABSTRACT: VHH stands for the variable regions of heavy chain only of camelid IgGs. The VHH family forms a set of interesting proteins derived from antibodies that maintain their capacity to recognize the antigen, despite their relatively small molecular weight (in the 12,000 Da range). Continuing our exploration of the possibilities of those molecules, we chose to design alternative molecules with maintained antigen recognition, but enhanced capacity, by fusing four VHH with one Fc, the fragment crystallizable region of antibodies. In doing so, we aimed at having a molecule with superior quantitative antigen recognition (×4) while maintaining its size below the 110 kDa. In the present paper, we described the building of those molecules that we coined VHH₂ -Fc-VHH₂ . The structu

SUBMITTER: Chabrol E 

PROVIDER: S-EPMC8376420 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature