Mixed xenogeneic porcine chimerism tolerizes human anti-pig natural antibody-producing cells in a humanized mouse model.

ABSTRACT:

Background

A major obstacle to the success of organ transplantation from pigs to humans, necessitated by the shortage of human organs, is robust humoral immune rejection by pig-reactive human antibodies. Mixed xenogeneic hematopoietic chimerism induces xenoreactive B cell tolerance in rodents, but whether mixed pig/human chimerism could induce tolerance of human B cells to pig xenoantigens is unknown.

Methods

We investigated this question using a humanized mouse model in which durable mixed (pig-human) xenogeneic chimerism can be established.

Results

Human natural anti-pig cytotoxic antibodies, predominantly IgM, are detectable in non-chimeric humanized mouse serum, and pig-reactive antibodies were reduced in mixed chimeric versus non-chimeric humanized mice. This dif

SUBMITTER: Waffarn EE 

PROVIDER: S-EPMC8376778 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature