Differential DNA methylation and mRNA transcription in gingival tissues in periodontal health and disease.

ABSTRACT:

Aim

We investigated differential DNA methylation in gingival tissues in periodontal health, gingivitis, and periodontitis, and its association with differential mRNA expression.

Materials and methods

Gingival tissues were harvested from individuals and sites with clinically healthy and intact periodontium, gingivitis, and periodontitis. Samples were processed for differential DNA methylation and mRNA expression using the IlluminaEPIC (850 K) and the IlluminaHiSeq2000 platforms, respectively. Across the three phenotypes, we identified differentially methylated CpG sites and regions, differentially expressed genes (DEGs), and genes with concomitant differential methylation at their promoters and expression were identified. The findings were validated using our earlier database

SUBMITTER: Kim H 

PROVIDER: S-EPMC8376779 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature