Co-expression of drug metabolizing cytochrome P450 enzymes and estrogen receptor alpha (ESR1) in human liver: racial differences and the regulatory role of ESR1.

ABSTRACT:

Objectives

The function and expression of cytochrome P450 (CYP) drug metabolizing enzymes is highly variable, greatly affecting drug exposure, and therapeutic outcomes. The expression of these enzymes is known to be controlled by many transcription factors (TFs), including ligand-free estrogen receptor alpha (ESR1, in the absence of estrogen). However, the relationship between the expression of ESR1, other TFs, and CYP enzymes in human liver is still unclear.

Methods

Using real-time PCR, we quantified the mRNA levels of 12 CYP enzymes and nine TFs in 246 human liver samples from European American (EA, n = 133) and African American (AA, n = 113) donors.

Results

Our results showed higher expression levels of ESR1 and six CYP enzymes in EA than in AA. Partial least squar

SUBMITTER: Collins JM 

PROVIDER: S-EPMC8376797 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature