Implications of zonal architecture on differential gene expression profiling and altered pathway expressions in mandibular condylar cartilage.

ABSTRACT: Mandibular condylar cartilage (MCC) is a multi-zonal heterogeneous fibrocartilage containing different types of cells, but the factors/mechanisms governing the phenotypic transition across the zones have not been fully understood. The reliability of molecular studies heavily rely on the procurement of pure cell populations from the heterogeneous tissue. We used a combined laser-capture microdissection and microarray analysis approach which allowed identification of differential zone-specific gene expression profiling and altered pathways in the MCC of 5-week-old rats. The bioinformatics analysis demonstrated that the MCC cells clearly exhibited distinguishable phenotypes from the articular chondrocytes. Additionally, a set of genes has been determined as potential markers to identify each

SUBMITTER: Basudan AM 

PROVIDER: S-EPMC8376865 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature