Personalized medicine for reconstruction of critical-size bone defects - a translational approach with customizable vascularized bone tissue.

ABSTRACT: Tissue engineering principles allow the generation of functional tissues for biomedical applications. Reconstruction of large-scale bone defects with tissue-engineered bone has still not entered the clinical routine. In the present study, a bone substitute in combination with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) with or without growth factors BMP-2 and VEGF-A was prevascularized by an arteriovenous (AV) loop and transplanted into a critical-size tibia defect in the sheep model. With 3D imaging and immunohistochemistry, we could show that this approach is a feasible and simple alternative to the current clinical therapeutic option. This study serves as proof of concept for using large-scale transplantable, vascularized, and customizable bone, generated in a li

SUBMITTER: Kengelbach-Weigand A 

PROVIDER: S-EPMC8377075 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature