In vitro antiplasmodial activity, cytotoxicity, and gas chromatography - flame ionization detector metabolites fingerprint of extracts and fractions from Tetrorchidium didymostemon.

ABSTRACT:

Background

Tetrorchidium didymostemon is used as an antimalarial remedy in southern Nigeria.

Objective(s)

This study was aimed at providing scientific validation for the use of T. didymostemon in the treatment of malaria in Nigeria.

Materials and methods

Plasmodium falciparum 3D7 (Pf3D7) strain was cultured and maintained in fresh O⁺ human erythrocytes. Standard methods were used to evaluate in vitro antiplasmodial activity, cytotoxic effect on Vero cell line, phytochemical screening, and antioxidant capacity. Gas Chromatography - Flame Ionization Detector (GC-FID) metabolite fingerprinting of the most potent fraction was carried out.

Results

The methanol leaf extract had higher antiplasmodial activity (IC₅₀Pf3D7 = 25 ± 0.21 μg/mL) in comp

SUBMITTER: Ebohon O 

PROVIDER: S-EPMC8377187 | biostudies-literature | 2021 Jul-Sep

REPOSITORIES: biostudies-literature