Sarcocystis cruzi infection in free-living European bison (Bison bonasus bonasus L.) from the Bialowieza Forest, Poland - A molecular analysis based on the cox1 gene.

ABSTRACT: European bison are susceptible to a range of pathogens which may influence their health, and hence, to ensure their protection, it is essential to provide effective monitoring of potential exposure. This study presents the first molecular confirmation of Sarcocystis cruzi infection in European bison based on PCR amplification of the cytochrome c oxidase subunit I (cox1) gene. A sample of heart tissue taken from one fifteen-year-old European bison cow was examined by light microscopy for the presence of heart sarcocysts. The genomic DNA isolated from any identified sarcocysts was subjected to PCR to amplify cox1 gene sequences, and the obtained amplicons were sequenced by Sanger dideoxy sequencing. Two partial cox1 sequences were obtained; they were identi

SUBMITTER: Cabaj W 

PROVIDER: S-EPMC8377485 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature