A Diffusion-like Process Accommodates New Crypts During Clonal Expansion in Human Colonic Epithelium.

ABSTRACT:

Background & aims

Colorectal cancer (CRC) is thought to arise when the cumulative mutational burden within colonic crypts exceeds a certain threshold that leads to clonal expansion and ultimately neoplastic transformation. Therefore, quantification of the fixation and subsequent expansion of somatic mutations in normal epithelium is key to understanding colorectal cancer initiation. The aim of the present study was to determine how advantaged expansions can be accommodated in the human colon.

Methods

Immunohistochemistry was used to visualize loss of the cancer driver KDM6A in formalin-fixed paraffin-embedded (FFPE) normal human colonic epithelium. Combining microscopy with neural network-based image analysis, we determined the frequencies of KDM6A-mutant crypts and fission/

SUBMITTER: Olpe C 

PROVIDER: S-EPMC8377717 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature