Harmonization of six quantitative SARS-CoV-2 serological assays using sera of vaccinated subjects.

ABSTRACT:

Background and aims

Vaccines, to limit SARS-CoV-2 infection, were produced and reliable assays are needed for their evaluation. The WHO produced an International-Standard (WHO-IS) to facilitate the standardization/comparison of serological methods. The WHO-IS, produced in limited amount, was never tested for reproducibility. This study aims at developing a reproducible and accessible working standard (WS) to complement the WHO-IS.

Materials and methods

Sera from vaccinated individuals were used to produce the WSs. The WHO-IS, the WSs and single serum samples (n = 48) were tested on 6 quantitative serological devices. Neutralization assays were performed for the 48 samples and compared with their antibody titers.

Results

The WS carry an antibody titer 20-fold higher th

SUBMITTER: Ferrari D 

PROVIDER: S-EPMC8378065 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature