Hypoxia triggers collective aerotactic migration in Dictyostelium discoideum.

ABSTRACT: Using a self-generated hypoxic assay, we show that the amoeba Dictyostelium discoideum displays a remarkable collective aerotactic behavior. When a cell colony is covered, cells quickly consume the available oxygen (O₂) and form a dense ring moving outwards at constant speed and density. To decipher this collective process, we combined two technological developments: porphyrin-based O₂ -sensing films and microfluidic O₂ gradient generators. We showed that Dictyostelium cells exhibit aerotactic and aerokinetic response in a low range of O₂ concentration indicative of a very efficient detection mechanism. Cell behaviors under self-generated or imposed O₂ gradients were modeled using an in silico cellular Potts model built o

SUBMITTER: Cochet-Escartin O 

PROVIDER: S-EPMC8378850 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature