Electrotaxis-on-Chip to Quantify Neutrophil Migration Towards Electrochemical Gradients.

ABSTRACT: Electric fields are generated in vivo in a variety of physiologic and pathologic settings, including wound healing and immune response to injuries to epithelial barriers (e.g. lung pneumocytes). Immune cells are known to migrate towards both chemical (chemotaxis), physical (mechanotaxis) and electric stimuli (electrotaxis). Electrotaxis is the guided migration of cells along electric fields, and has previously been reported in T-cells and cancer cells. However, there remains a need for engineering tools with high spatial and temporal resolution to quantify EF guided migration. Here we report the development of an electrotaxis-on-chip (ETOC) platform that enables the quantification of dHL-60 cell, a model neutrophil-like cell line, migration toward both electrical and chemoattractant

SUBMITTER: Moarefian M 

PROVIDER: S-EPMC8379007 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature