Phosphorylation of CrkL S114 induced by common gamma chain cytokines and T-cell receptor signal transduction.

ABSTRACT: T-cell activation and cellular expansion by common gamma chain cytokines such as Interleukin-2 is necessary for adaptive immunity. However, when unregulated these same pathways promote pathologies ranging from autoimmune disorders to cancer. While the functional role of Interleukin-2 and downstream effector molecules is relatively clear, the repertoire of phosphoregulatory proteins downstream of this pathway is incomplete. To identify phosphoproteins downstream of common gamma chain receptor, YT cells were radiolabeled with [³²P]-orthophosphate and stimulated with Interleukin-2. Subsequently, tyrosine phosphorylated proteins were immunopurified and subjected to tandem mass spectrometry-leading to the identification of CrkL. Phosphoamino acid analysis revealed concurrent serine p

SUBMITTER: Estrada A 

PROVIDER: S-EPMC8379229 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature