Cell cycle induction in human cardiomyocytes is dependent on biosynthetic pathway activation.

ABSTRACT:

Aims

The coordinated gene and metabolic programs that facilitate cardiomyocyte entry and progression in the cell cycle are poorly understood. The purpose of this study was to identify the metabolic changes that influence myocyte proliferation.

Methods and results

In adult mouse cardiomyocytes and human induced pluripotent stem cell cardiomyocytes (hiPS-CMs), cell cycle initiation by ectopic expression of Cyclin B1, Cyclin D1, CDK1, and CDK4 (termed 4F) downregulated oxidative phosphorylation genes and upregulated genes that regulate ancillary biosynthetic pathways of glucose metabolism. Results from metabolic analyses and stable isotope tracing experiments indicate that 4F-mediated cell cycle induction in hiPS-CMs decreases glucose oxidation and oxidative phosphorylation and

SUBMITTER: Abouleisa RRE 

PROVIDER: S-EPMC8379496 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature