Semicoordinated allelic-bursting shape dynamic random monoallelic expression in pregastrulation embryos.

ABSTRACT: Recently, allele-specific single-cell RNA-seq analysis has demonstrated widespread dynamic random monoallelic expression of autosomal genes (aRME) in different cell types. However, the prevalence of dynamic aRME during pregastrulation remains unknown. Here, we show that dynamic aRME is widespread in different lineages of pregastrulation embryos. Additionally, the origin of dynamic aRME remains elusive. It is believed that independent transcriptional bursting from each allele leads to dynamic aRME. Here, we show that allelic burst is not perfectly independent; instead it happens in a semicoordinated fashion. Importantly, we show that semicoordinated allelic bursting of genes, particularly with low burst frequency, leads to frequent asynchronous allelic bursting, thereby contributing to dyna

SUBMITTER: Naik HC 

PROVIDER: S-EPMC8379509 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature