Ablation of glucokinase-expressing tanycytes impacts energy balance and increases adiposity in mice.

ABSTRACT:

Objectives

Glucokinase (GCK) is critical for glucosensing. In rats, GCK is expressed in hypothalamic tanycytes and appears to play an essential role in feeding behavior. In this study, we investigated the distribution of GCK-expressing tanycytes in mice and their role in the regulation of energy balance.

Methods

In situ hybridization, reporter gene assay, and immunohistochemistry were used to assess GCK expression along the third ventricle in mice. To evaluate the impact of GCK-expressing tanycytes on arcuate neuron function and mouse physiology, Gck deletion along the ventricle was achieved using loxP/Cre recombinase technology in adult mice.

Results

GCK expression was low along the third ventricle, but detectable in tanycytes facing the ventromedial arcuate nucleus

SUBMITTER: Rohrbach A 

PROVIDER: S-EPMC8379510 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature