Clinical and epidemiologic characteristics of inconclusive results in SARS-CoV-2 RT-PCR assays.

ABSTRACT:

Background

Inconclusive results in SARS-CoV-2 molecular assays cause confusion among clinicians and delay appropriate infection prevention and control. In this study, we aimed to characterize the respiratory specimens associated with inconclusive SARS-CoV-2 molecular assay results.

Methods

We re-evaluated inconclusive specimens by 3 additional RT-PCR assays and attempted to detect subgenomic RNA (sgRNA) in these specimens.

Results

Among follow-up tests from confirmed SARS-CoV-2 cases, 36.3% of the inconclusive results were classified as presumptive positive results (45/124). However, none of the specimens from 36 screening cases was classified as a presumptive positive result. Among 160 inconclusive specimens, sgRNAs were detected in 78 samples (48.8%): 58 were confir

SUBMITTER: Lim YK 

PROVIDER: S-EPMC8379569 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature