ITRAQ-based quantitative proteomic analysis of thoracic aortas from adult rats born to preeclamptic dams.

ABSTRACT:

Background

Preeclampsia and gestational hypertension can cause vascular function impairment in offspring. In our previous work, we described the protein expression profiles of umbilical artery tissues from patients with preeclampsia.

Methods

To gain insights into the mechanisms of vascular dysfunction in adult rats born to preeclamptic dams, we analyzed thoracic aorta tissues by using iTRAQ isobaric tags and 2D nano LC-MS/MS.

Results

By using the iTRAQ method, we analyzed 1825 proteins, of which 106 showed significantly different expression in the thoracic aortic. Ingenuity pathway analysis (IPA) showed that the majority of differentially expressed proteins (DEPs) were associated with cardiovascular function. Further analysis indicated that glucose-6-phosphate dehydro

SUBMITTER: Yu B 

PROVIDER: S-EPMC8379584 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature