Nonrandom segregation of sister chromosomes by <i>Escherichia coli</i> MukBEF.

ABSTRACT: Structural maintenance of chromosomes (SMC) complexes contribute to chromosome organization in all domains of life. In Escherichia coli, MukBEF, the functional SMC homolog, promotes spatiotemporal chromosome organization and faithful chromosome segregation. Here, we address the relative contributions of MukBEF and the replication terminus (ter) binding protein, MatP, to chromosome organization-segregation. We show that MukBEF, but not MatP, is required for the normal localization of the origin of replication to midcell and for the establishment of translational symmetry between newly replicated sister chromosomes. Overall, chromosome orientation is normally maintained through division from one generation to the next. Analysis of loci flanking the replication termination regio

SUBMITTER: Makela J 

PROVIDER: S-EPMC8379921 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature