Absence of carbonic anhydrase in chloroplasts affects C₃ plant development but not photosynthesis.

ABSTRACT: The enzyme carbonic anhydrase (CA), which catalyzes the interconversion of bicarbonate with carbon dioxide (CO₂) and water, has been hypothesized to play a role in C₃ photosynthesis. We identified two tobacco stromal CAs, β-CA1 and β-CA5, and produced CRISPR/Cas9 mutants affecting their encoding genes. While single knockout lines Δβ-ca1 and Δβ-ca5 had no striking phenotypic differences compared to wild type (WT) plants, Δβ-ca1ca5 leaves developed abnormally and exhibited large necrotic lesions even when supplied with sucrose. Leaf development of Δβ-ca1ca5 plants normalized at 9,000 ppm CO₂ Leaves of Δβ-ca1ca5 mutants and WT that had matured in high CO₂ had identical CO₂ fix

SUBMITTER: Hines KM 

PROVIDER: S-EPMC8379964 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature