Characterizing parasitic nematode faunas in faeces and soil using DNA metabarcoding.

ABSTRACT:

Background

Gastrointestinal parasitic nematodes can impact fecundity, development, behaviour, and survival in wild vertebrate populations. Conventional monitoring of gastrointestinal parasitic nematodes in wild populations involves morphological identification of eggs, larvae, and adults from faeces or intestinal samples. Adult worms are typically required for species-level identification, meaning intestinal material from dead animals is needed to characterize the nematode community with high taxonomic resolution. DNA metabarcoding of environmental samples is increasingly used for time- and cost-effective, high-throughput biodiversity monitoring of small-bodied organisms, including parasite communities. Here, we evaluate the potential of DNA metabarcoding of faeces and soil samples

SUBMITTER: Davey ML 

PROVIDER: S-EPMC8380370 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature