RNA sequencing reveals niche gene expression effects of beta-hydroxybutyrate in primary myotubes.

ABSTRACT: Various forms of fasting and ketogenic diet have shown promise in (pre-)clinical studies to normalize body weight, improve metabolic health, and protect against disease. Recent studies suggest that β-hydroxybutyrate (βOHB), a fasting-characteristic ketone body, potentially acts as a signaling molecule mediating its beneficial effects via histone deacetylase inhibition. Here, we have investigated whether βOHB, in comparison to the well-established histone deacetylase inhibitor butyrate, influences cellular differentiation and gene expression. In various cell lines and primary cell types, millimolar concentrations of βOHB did not alter differentiation in vitro, as determined by gene expression and histological assessment, whereas equimolar concentrations of butyrate consistently impaired dif

SUBMITTER: Ruppert PM 

PROVIDER: S-EPMC8380668 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature