Optogenetic Control of the Canonical Wnt Signaling Pathway During Xenopus laevis Embryonic Development.

ABSTRACT: Optogenetics uses light-inducible protein-protein interactions to precisely control the timing, localization, and intensity of signaling activity. The precise spatial and temporal resolution of this emerging technology has proven extremely attractive to the study of embryonic development, a program faithfully replicated to form the same organism from a single cell. We have previously performed a comparative study for optogenetic activation of receptor tyrosine kinases, where we found that the cytoplasm-to-membrane translocation-based optogenetic systems outperform the membrane-anchored dimerization systems in activating the receptor tyrosine kinase signaling in live Xenopus embryos. Here, we determine if this engineering strategy can be generalized to other signaling pathways involving mem

SUBMITTER: Krishnamurthy VV 

PROVIDER: S-EPMC8380706 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature