Systematic functional interrogation of human pseudogenes using CRISPRi.

ABSTRACT:

Background

The human genome encodes over 14,000 pseudogenes that are evolutionary relics of protein-coding genes and commonly considered as nonfunctional. Emerging evidence suggests that some pseudogenes may exert important functions. However, to what extent human pseudogenes are functionally relevant remains unclear. There has been no large-scale characterization of pseudogene function because of technical challenges, including high sequence similarity between pseudogene and parent genes, and poor annotation of transcription start sites.

Results

To overcome these technical obstacles, we develop an integrated computational pipeline to design the first genome-wide library of CRISPR interference (CRISPRi) single-guide RNAs (sgRNAs) that target human pseudogene promoter-proxima

SUBMITTER: Sun M 

PROVIDER: S-EPMC8381491 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature