Saliva and Serum Protein Adsorption on Chemically Modified Silica Surfaces.

ABSTRACT: Biomaterials, once inserted in the oral cavity, become immediately covered by a layer of adsorbed proteins that consists mostly of salivary proteins but also of plasma proteins if the biomaterial is placed close to the gingival margin or if it becomes implanted into tissue and bone. It is often this protein layer, rather than the pristine biomaterial surface, that is subsequently encountered by colonizing bacteria or attaching tissue cells. Thus, to study this important initial protein adsorption from human saliva and serum and how it might be influenced through chemical modification of the biomaterial surface, we have measured the amount of protein adsorbed and analyzed the composition of the adsorbed protein layer using gel electrophoresis and western blotting. Here, we have developed an

SUBMITTER: Lehnfeld J 

PROVIDER: S-EPMC8381597 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature