Redefining Protein Interfaces within Protein Single Crystals with DNA.

ABSTRACT: Proteins are exquisite nanoscale building blocks: molecularly pure, chemically addressable, and inherently selective for their evolved function. The organization of proteins into single crystals with high positional, orientational, and translational order results in materials where the location of every atom can be known. However, controlling the organization of proteins is challenging due to the myriad interactions that define protein interfaces within native single crystals. Recently, we discovered that introducing a single DNA-DNA interaction between protein surfaces leads to changes in the packing of proteins within single crystals and the protein-protein interactions (PPIs) that arise. However, modifying specific PPIs to effect deliberate changes to protein packing is an unmet challen

SUBMITTER: Partridge BE 

PROVIDER: S-EPMC8381744 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature