Cost-Effective Mapping of Genetic Interactions in Mammalian Cells.

ABSTRACT: Comprehensive maps of genetic interactions in mammalian cells are daunting to construct because of the large number of potential interactions, ~ 2 × 10⁸ for protein coding genes. We previously used co-inheritance of distant genes from published radiation hybrid (RH) datasets to identify genetic interactions. However, it was necessary to combine six legacy datasets from four species to obtain adequate statistical power. Mapping resolution was also limited by the low density PCR genotyping. Here, we employ shallow sequencing of nascent human RH clones as an economical approach to constructing interaction maps. In this initial study, 15 clones were analyzed, enabling construction of a network with 225 genes and 2,359 interactions (FDR < 0.05). Despite its small size, the network sh

SUBMITTER: Khan AH 

PROVIDER: S-EPMC8381747 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature