Simple and efficient heterologous expression of necrosis-inducing effectors using the model plant Nicotiana benthamiana.

ABSTRACT: Plant fungal pathogens cause devastating diseases on cereal plants and threaten global food security. During infection, these pathogens secrete proteinaceous effectors that promote disease. Some of these effectors from necrotrophic plant pathogens induce a cell death response (necrosis), which facilitates pathogen growth in planta. Characterization of these effectors typically requires heterologous expression, and microbial expression systems such as bacteria and yeast are the predominantly used. However, microbial expression systems often require optimization for any given effector and are, in general, not suitable for effectors involving cysteine bridges and posttranslational modifications for activity. Here, we describe a simple and efficient method for expressing such effectors

SUBMITTER: Dagvadorj B 

PROVIDER: S-EPMC8381856 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature