Measuring Lipid Transfer Protein Activity Using Bicelle-Dilution Model Membranes.

ABSTRACT: In vitro assessment of lipid intermembrane transfer activity by cellular proteins typically involves measurement of either radiolabeled or fluorescently labeled lipid trafficking between vesicle model membranes. Use of bilayer vesicles in lipid transfer assays usually comes with inherent challenges because of complexities associated with the preparation of vesicles and their rather short "shelf life". Such issues necessitate the laborious task of fresh vesicle preparation to achieve lipid transfer assays of high quality, precision, and reproducibility. To overcome these limitations, we have assessed model membrane generation by bicelle dilution for monitoring the transfer rates and specificity of various BODIPY-labeled sphingolipids by different glycolipid transfer protein (GLTP) superfami

SUBMITTER: Gao YG 

PROVIDER: S-EPMC8382047 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature