Vascular deficiencies in renal organoids and ex vivo kidney organogenesis.

ABSTRACT: Chronic kidney disease (CKD) and end stage renal disease (ESRD) are increasingly frequent and devastating conditions that have driven a surge in the need for kidney transplantation. A stark shortage of organs has fueled interest in generating viable replacement tissues ex vivo for transplantation. One promising approach has been self-organizing organoids, which mimic developmental processes and yield multicellular, organ-specific tissues. However, a recognized roadblock to this approach is that many organoid cell types fail to acquire full maturity and function. Here, we comprehensively assess the vasculature in two distinct kidney organoid models as well as in explanted embryonic kidneys. Using a variety of methods, we show that while organoids can develop a wide range of kidney cell type

SUBMITTER: Ryan AR 

PROVIDER: S-EPMC8382085 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature