In Situ Classification of Cell Types in Human Kidney Tissue Using 3D Nuclear Staining.

ABSTRACT: To understand the physiology and pathology of disease, capturing the heterogeneity of cell types within their tissue environment is fundamental. In such an endeavor, the human kidney presents a formidable challenge because its complex organizational structure is tightly linked to key physiological functions. Advances in imaging-based cell classification may be limited by the need to incorporate specific markers that can link classification to function. Multiplex imaging can mitigate these limitations, but requires cumulative incorporation of markers, which may lead to tissue exhaustion. Furthermore, the application of such strategies in large scale 3-dimensional (3D) imaging is challenging. Here, we propose that 3D nuclear signatures from a DNA stain, DAPI, which could be incorporated in m

SUBMITTER: Woloshuk A 

PROVIDER: S-EPMC8382162 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature