Intestinal organoid-based 2D monolayers mimic physiological and pathophysiological properties of the pig intestine.

ABSTRACT: Gastrointestinal infectious diseases remain an important issue for human and animal health. Investigations on gastrointestinal infectious diseases are classically performed in laboratory animals leading to the problem that species-specific models are scarcely available, especially when it comes to farm animals. The 3R principles of Russel and Burch were achieved using intestinal organoids of porcine jejunum. These organoids seem to be a promising tool to generate species-specific in vitro models of intestinal epithelium. 3D Organoids were grown in an extracellular matrix and characterized by qPCR. Organoids were also seeded on permeable filter supports in order to generate 2D epithelial monolayers. The organoid-based 2D monolayers were characterized morphologically and were investigated re

SUBMITTER: Hoffmann P 

PROVIDER: S-EPMC8382199 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature