Scanning Ion Conductance Microscopy Study Reveals the Disruption of the Integrity of the Human Cell Membrane Structure by Oxidative DNA Damage.

ABSTRACT: Oxidative stress can damage organs, tissues, and cells through reactive oxygen species (ROS) by oxidizing DNA, proteins, and lipids, thereby resulting in diseases. However, the underlying molecular mechanisms remain to be elucidated. In this study, employing scanning ion conductance microscopy (SICM), we explored the early responses of human embryonic kidney (HEK293H) cells to oxidative DNA damage induced by potassium chromate (K₂CrO₄). We found that the short term (1-2 h) exposure to a low concentration (10 μM) of K₂CrO₄ damaged the lipid membrane of HEK293H cells, resulting in structural defects and depolarization of the cell membrane and reducing cellular secretion activity shortly after the treatment. We further demonstrated that the K

SUBMITTER: Rubfiaro AS 

PROVIDER: S-EPMC8382241 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature