Cesium based phasing of macromolecules: a general easy to use approach for solving the phase problem.

ABSTRACT: Over the last decades the phase problem in macromolecular x-ray crystallography has become more controllable as methods and approaches have diversified and improved. However, solving the phase problem is still one of the biggest obstacles on the way of successfully determining a crystal structure. To overcome this caveat, we have utilized the anomalous scattering properties of the heavy alkali metal cesium. We investigated the introduction of cesium in form of cesium chloride during the three major steps of protein treatment in crystallography: purification, crystallization, and cryo-protection. We derived a step-wise procedure encompassing a "quick-soak"-only approach and a combined approach of CsCl supplement during purification and cryo-protection. This procedure was successfully applie

SUBMITTER: Koelmel W 

PROVIDER: S-EPMC8382735 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature