Retinoic Acid Induces Functionally Suppressive Foxp3⁺RORγt⁺ T Cells In Vitro.

ABSTRACT:

Introduction

CD4⁺ T cells with regulatory function co-expressing Foxp3 and RORγt are linked to the development of oral tolerance towards innocuous food antigens in mice. This study aimed to discern the role played by IL-6 and retinoic acid (RA) in the in vitro generation of Foxp3⁺RORγt⁺ T cells and to investigate whether such cells have suppressive properties.

Methods

CD4⁺CD25^- T cells isolated from the spleen of BALB/c mice, were stimulated in the presence of IL-2 alone or together with TFG-β and different concentrations of IL-6 and/or RA. Percentage of Foxp3⁺, RORγt⁺, IL-17⁺, Foxp3⁺RORγt^-, Foxp3⁺RORγt⁺, and Foxp3^-RORγt^+<

SUBMITTER: Martinez-Blanco M 

PROVIDER: S-EPMC8382797 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature