Visualization of Mineral-Targeted Alkaline Phosphatase Binding to Sites of Calcification In Vivo.

ABSTRACT: A mineral-targeted form of recombinant tissue-nonspecific alkaline phosphatase (TNAP), asfotase alfa, was approved multinationally as an enzyme replacement therapy for hypophosphatasia in 2015. Two reports to date have shown evidence of binding of this drug to mineralizing tissues using histochemistry and immunohistochemistry. Here, we sought to expand on those earlier studies by directly visualizing the in vivo binding of asfotase alfa conjugated with AnaTag HiLyte Fluor 750 or Alexa Fluor 647 fluorescent dye to sites of skeletal/dental mineralization and ectopic calcification. We utilized 40-day-old Tagln-Cre; Hprt^ALPL/Y mice, a model of severe medial vascular calcification; Tie2-Cre; Hprt^ALPL/Y mice, a model of severe intimal calcification; and sibling WT Hprt

SUBMITTER: Amadeu de Oliveira F 

PROVIDER: S-EPMC8383212 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature