Design of Nucleic Acid Biosensors Based on CRISPR/Cas Systems and Reporter Split Proteins.

ABSTRACT: Highly sensitive, specific, rapid, and easy-to-use diagnostic methods for the detection of nucleic acids of pathogens are required for the diagnosis of many human, animal, and plant diseases and environmental monitoring. The approaches based on the use of the natural ability of bacterial CRISPR/Cas9 systems to recognize DNA sequences with a high specificity under isothermal conditions are an alternative to the polymerase chain reaction method, which requires expensive laboratory equipment. The development of the methods for signal registration with the formation of a DNA/RNA/Cas9 protein complex is a separate bioengineering task. In this work, a design was developed and the applicability of a biosensor system based on the binding of two dCas9 proteins with target DNA sequences (without the

SUBMITTER: Novikov RV 

PROVIDER: S-EPMC8383255 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature