Effect of Nutritional Determinants and TonB on the Natural Transformation of Riemerella anatipestifer.

ABSTRACT: Riemerella anatipestifer is a gram-negative bacterium that is the first naturally competent bacterium identified in the family Flavobacteriaceae. However, the determinants that influence the natural transformation and the underlying mechanism remain unknown. In this study, we evaluated the effects of various nutritional factors of the GCB medium [glucose, L-glutamine, vitamin B1, Fe (NO₃)₃, NaCl, phosphate, and peptone], on the natural transformation of R. anatipestifer ATCC 11845. Among the assayed nutrients, peptone and phosphate affected the natural transformation of R. anatipestifer ATCC 11845, and the transformation frequency was significantly decreased when phosphate or peptone was removed from the GCB medium. When the iron chelator 2

SUBMITTER: Zhang L 

PROVIDER: S-EPMC8383284 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature