Serum Exosomal Circular RNA Expression Profile and Regulative Role in Proliferative Diabetic Retinopathy.

ABSTRACT:

Background

Proliferative diabetic retinopathy (PDR), as one of the main microvascular complications of diabetes mellitus, seriously threatens the visual function of the working-age population; yet, the underlying pathogenesis is still poorly understood. This study aimed to identify the distinct exosomal circular RNA (circRNA) expression in PDR serum and preliminarily explore the potential pro-angiogenic mechanism of specific exosomal circRNAs.

Methods

We collected serum samples from 10 patients with PDR and 10 patients with age-matched senile cataract to detect the exosomal differentially expressed genes (DEGs) of circRNAs via high-throughput sequencing, followed by validation with quantitative real-time PCR (qRT-PCR). Next, bioinformatics analyses including competiti

SUBMITTER: Li X 

PROVIDER: S-EPMC8383346 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature