Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.

ABSTRACT:

Background

Endometriosis is a serious reproductive and general health consequences. Recombinant human IL-37 (rhIL-37) is an inhibitor of inflammation.

Methods

ELISA assay was performed to detect the concentration of cytokines. Flow cytometry was used to analyze cell proportion. Besides, qRT-PCR and western blotting assay were used to detect the level of gene and protein, respectively. Transwell co-culture system was used for the co-culture of dendritic cells (DCs) and CD4⁺T cells.

Results

Our data showed that rhIL-37 inhibited the development of ectopic lesions in the mice with endometriosis, increased Th1/Th2 ratio and induced DCs maturation. The co-culture system of DCs and CD4⁺T cells demonstrated that rhIL-37 increased Th1/Th2 cell ratio thro

SUBMITTER: Li L 

PROVIDER: S-EPMC8383354 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature