Norepinephrine modulates IL-1β-induced catabolic response of human chondrocytes.

ABSTRACT:

Background

The influence of the sympathetic nervous system (SNS) on metabolism of bone and cartilage expressing β-adrenergic receptors (AR) was suggested. Here, we investigated whether the SNS functions as a modulator of cartilage metabolism induced by interleukin-1beta (IL-1β).

Methods

Human articular chondrocytes and articular cartilage were collected from patients with osteoarthritis (OA). Chondrocyte monolayer and cartilage explant culture were stimulated with IL-1β. The activity of β-ARs was modulated by an agonist, norepinephrine (NE), and antagonists, including propranolol, atenolol, nebivolol, and nadolol.

Results

The levels of β₁-, β₂-, and β₃-AR in OA cartilage and IL-1β-treated chondrocytes were lower than normal cartilage a

SUBMITTER: Hwang HS 

PROVIDER: S-EPMC8383445 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature