Immune Monitoring Assay for Extracorporeal Photopheresis Treatment Optimization After Heart Transplantation.

ABSTRACT:

Background

Extracorporeal photopheresis (ECP) induces immunological changes that lead to a reduced risk of transplant rejection. The aim of the present study was to determine optimum conditions for ECP treatment by analyzing a variety of tolerance-inducing immune cells to optimize the treatment.

Methods

Ten ECP treatments were applied to each of 17 heart-transplant patients from month 3 to month 9 post-HTx. Blood samples were taken at baseline, three times during treatment, and four months after the last ECP treatment. The abundance of subsets of tolerance-inducing regulatory T cells (T_regs) and dendritic cells (DCs) in the samples was determined by flow cytometry. A multivariate statistical model describing the immunological status of rejection-free heart transpl

SUBMITTER: Dieterlen MT 

PROVIDER: S-EPMC8383491 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature