Downregulation of KLF4 activates embryonic and fetal globin mRNA expression in human erythroid progenitor cells.

ABSTRACT: The Krüppel-like factor (KLF) family dominates highly conserved three zinc finger DNA binding domains at the C-terminus and variable transactivation domains at the N-terminus. Humans possess 18 KLF genes that are differentially expressed in various tissues. Several KLFs recognize a specific CACCC DNA motif that is commonly found within hematopoietic-specific promoters. To investigate those KLFs that are involved in human hemoglobin (Hb) switching, the present study analyzed a previous microarray data set from fetal and adult erythroid cells and validated the mRNA expression levels of 18 KLFs by reverse transcription-quantitative PCR (RT-qPCR). KLF with a decreased expression level in the fetuses was selected for a functional study in human erythroid progenitor cells us

SUBMITTER: Khamphikham P 

PROVIDER: S-EPMC8383760 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature