Short-Term High Fructose Intake Impairs Diurnal Oscillations in the Murine Cornea.

ABSTRACT:

Purpose

Endogenous and exogenous stressors, including nutritional challenges, may alter circadian rhythms in the cornea. This study aimed to determine the effects of high fructose intake (HFI) on circadian homeostasis in murine cornea.

Methods

Corneas of male C57BL/6J mice subjected to 10 days of HFI (15% fructose in drinking water) were collected at 3-hour intervals over a 24-hour circadian cycle. Total extracted RNA was subjected to high-throughput RNA sequencing. Rhythmic transcriptional data were analyzed to determine the phase, rhythmicity, unique signature, metabolic pathways, and cell signaling pathways of transcripts with temporally coordinated expression. Corneas of HFI mice were collected for whole-mounted techniques after immunofluorescent staining to quantify mit

SUBMITTER: He J 

PROVIDER: S-EPMC8383902 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature