Loss of <i>N</i>¹-methylation of G37 in tRNA induces ribosome stalling and reprograms gene expression.

ABSTRACT: N¹-methylation of G37 is required for a subset of tRNAs to maintain the translational reading-frame. While loss of m¹G37 increases ribosomal +1 frameshifting, whether it incurs additional translational defects is unknown. Here, we address this question by applying ribosome profiling to gain a genome-wide view of the effects of m¹G37 deficiency on protein synthesis. Using E coli as a model, we show that m¹G37 deficiency induces ribosome stalling at codons that are normally translated by m¹G37-containing tRNAs. Stalling occurs during decoding of affected codons at the ribosomal A site, indicating a distinct mechanism than that of +1 frameshifting, which occurs after the affected codons leave the A site. Enzyme- and cell-bas

SUBMITTER: Masuda I 

PROVIDER: S-EPMC8384417 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature